All lanes : Anti-Tristetraprolin antibody (ab83579) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell LysateLane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 6 : SK N SH (Human neuroblastoma) Whole Cell LysateLane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.