Anti-TRK fused gene antibody
| Name | Anti-TRK fused gene antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab86606 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rabbit, Horse, Guinea Pig, Bovine, Dog, Pig, Chimpanzee, Ferret, Monkey, Orangutan, Elephant |
| Antigen | Synthetic peptide, corresponding to region between residues 175 and 225 of human TRK fused gene (NP_001007566 |
| Description | Rabbit Polyclonal |
| Gene | TFG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-TRK fused gene antibody (ab86606) at 1/0.1 dilutionLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µg
Detection of TRK fused gene by Western Blot of Immunprecipitate. ab86606 at 1µg/ml staining TRK fused gene in HeLa whole cell lysate immunoprecipitated using ab86606 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
ICC/IF image of ab86606 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86606, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.