All lanes : Anti-TrkB (phospho Y515) antibody (ab109684) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with Immunizing peptide at 1 µg/mlLane 4 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188) with Immunizing peptide at 1 µg/mlLane 5 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466) with Control peptide at 1 µg/mlLane 6 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188) with Control peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab109684 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.