Anti-TRP2 antibody
| Name | Anti-TRP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab74073 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthesized peptide derived from an internal region of human TRP2 |
| Description | Rabbit Polyclonal |
| Gene | DCT |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab74073 staining TRP2 in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
All lanes : Anti-TRP2 antibody (ab74073) at 1/500 dilutionLane 1 : Extracts from K562 cells with no peptideLane 2 : Extracts from K562 cells with peptide at 5 µgLysates/proteins at 5 µg per lane.
Immunofluorescence analysis of TRP2 in A549 cells, using ab74073 at a dilution of 1/500. Left hand panel: no peptide right hand panel: with immunising peptide.
ab74043 staining TRP2 in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using a TRIS/EDTA buffer pH 9.0. Samples were then permeabilized, followed by incubation with the primary antibody at a 1/1000 dilution for 30 minutes at 20°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.
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