All lanes : Anti-TRP2 antibody (ab92995) at 1 µg/mlLane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-TRP2 antibody (ab92995) at 1/1000 dilutionLane 1 : Human A375 whole cell lysate with 21% OxygenLane 2 : Human A375 whole cell lysate with 1% OxygenLysates/proteins at 10000 cells per lane.SecondaryHRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.