All lanes : Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Human fetal liver lysateLane 3 : SK-OV-3 cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-TSC22 domain family, member 4 antibody [EPR14789] - C-terminal (ab190349) at 1/1000 dilutionLane 1 : mouse RAW 264.7 cell lysateLane 2 : rat PC12 cell lysateLane 3 : mouse NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Western blot analysis on immunoprecipitation from 1) HeLa cell lysate and 2) PBS, labeling TSC22 domain family, member 4 using ab190349 at 1/50 dilution and HRP-conjugated anti-rabbit IgG specific to the non-reduced form of rabbit IgG at a 1/1500 dilution.
Flow cytometry analysis of paraformaldehyde-fixed HeLa cells labeling TSC22 domain family, member 4 with ab190349 at a 1/50 dilution (pink) or a rabbit IgG isotype control (green), followed Goat anti rabbit IgG (FITC) at a 1/150 dilution.