All lanes : Anti-TSSK4 antibody - C-terminal (ab170349) at 1/100 dilutionLane 1 : Non-transfected 293 cell lysatesLane 2 : 293 cell lysates transfected with TSSK4Lysates/proteins at 2 µg per lane.
Immunohistochemistry analysis of formalin-fixed paraffin-embedded Human testis tissue labeling TSSK4 with ab170349 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining .
Immunofluorescence analysis of 293 cells labeling TSSK4 with ab170349 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).