All lanes : Anti-TTC4 antibody [EPR13381] (ab181194) at 1/50000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TTC4 antibody [EPR13381] (ab181194) at 1/10000 dilution + MCF7 at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling TTC4 with ab181194 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TTC4 with ab181194 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181194 at 1/200 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®488). Conterstained with Dapi (blue).
Western blot analysis of TTC4 in immunoprecipitation pellets from 293T cell lysate.Lane 1: ab181194 used at 1/50 dilution. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Lane 2: Negative control