All lanes : Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/20000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : U87-MG cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/5000 dilution + MCF7 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TTC4 with ab181195 at 1/100 dilution. Secondary antibody: anti rabbit IgG (HRP), prediluted. Counter stain: Hematoxylin.
Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181195 at 1/250 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor® 488) at a 1/200 dilution. Conterstained with Dapi (blue).