Lane 1 : Anti-TTC5 antibody (ab36855) at 0.5 µg/mlLane 2 : Anti-TTC5 antibody (ab36855) at 1 µg/mlLane 3 : Anti-TTC5 antibody (ab36855) at 2 µg/mlLane 1 : RAW264.7 cell lysate Lane 2 : RAW264.7 cell lysate Lane 3 : RAW264.7 cell lysate Lysates/proteins at 15 µg/ml per lane.Secondaryanti-rabbit IgG HRP at 1/10000 dilution
ab36855 at 10µg/ml staining TCC5 in mouse brain tissue by IHC
ICC/IF image of ab36855 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor®594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of TTC5 in Mouse Brain cells using ab36855 at 20 ug/ml.