Phospho-Cyclin D1 (Thr286) (D29B3) XP ® Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 3300
• Prices: $119.00, $318.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D29B3
• Applications: WB IP ICC/IF FC
• Species Reactivities: Human, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr286 of cyclin D1.
• Description: Rabbit Monoclonal
• Gene: CCND1

Product images

Western blot analysis of extracts from HT-1080 cells, treated with MG132 and with or without λ phosphatase, using Phospho-Cyclin D1 (Thr286) (D29B3) XP ® Rabbit mAb (upper) or Cyclin D1 (DC56) Mouse mAb #2926 (lower).

Western blot analysis of extracts from HT-1080 cells, untreated or treated with MG132 (10 μM, 4 hours), using Phospho-Cyclin D1 (Thr286) (D29B3) XP ® Rabbit mAb.

Confocal immmunofluorescent analysis of HT-1080 (top) and Saos-2 cells (bottom), untreated (left) or treated with MG132 alone (center) or with MG132 followed by λ-phosphatase (right), using Phospho-Cyclin D1 (Thr286) (D29B3) XP ® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Flow cytometric analysis of HT-1080 cells, untreated (blue) or MG132-treated (green), using Phospho-Cyclin D1 (Thr286) (D29B3) XP ® Rabbit mAb.