Anti-TUBB2A antibody
| Name | Anti-TUBB2A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92857 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1 - 446 of Human TUBB2A (AAH01194), with a proprietary tag |
| Description | Mouse Monoclonal |
| Gene | TUBB2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab92857 stained SW480 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92857 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Overlay histogram showing Jurkat cells stained with ab92857 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92857, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/16233_ab92857-6-ab92857FC.jpg)
Overlay histogram showing Jurkat cells stained with ab92857 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92857, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-TUBB2A antibody (ab92857) at 5 µg/ml + PC12 cell lysate at 50 µgSecondaryHRP conjugated Goat anti-Mouse IgG at 1/2500 dilution
Anti-TUBB2A antibody (ab92857) at 5 µg/ml + NIH 3T3 cell lysate at 50 µgSecondary HRP conjugated Goat anti-Mouse IgG at 1/2500 dilution
Anti-TUBB2A antibody (ab92857) at 5 µg/ml + Jurkat cell lysate at 50 µgSecondaryHRP conjugated Goat anti-Mouse IgG at 1/2500 dilution
ab92857 at 6µg/ml staining TUBB2A in formalin-fixed, paraffin-embedded Human colon tissue.