All lanes : Anti-TUBB2A antibody [EPR11843] (ab170931) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HepG2 cell lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugate at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TUBB2A with ab170931 at 1/100 dilution.
Western blot analysis on immunoprecipitation pellet from 293T cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170931 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.Lane 1 Primary: ab170931, 1/10000 dilutionSample: Immunoprecipitation pellet from 293T cell lysateSecondary: HRP-conjugated anti-rabbit IgG Lane 2 Primary: ab170931, 1/10000 dilutionSample: 1X PBS (negative control)Secondary: HRP-conjugated anti-rabbit IgG
Flow cytometric analysis of permeabilized Jurkat cells labeling TUBB2A with ab170931 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).