All lanes : Anti-TUFM antibody [EPR12796(B)] (ab175199) at 1/10000 dilutionLane 1 : HepG2 cell lysatesLane 2 : A431 cell lysatesLane 3 : HeLa cell lysatesLane 4 : Jurkat cell lysatesLysates/proteins at 10 µg per lane.
Immunofluorescence analysis of HepG2 cells labeling TUFM with ab175199 at a 1/50 dilution (green). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cells lysate or (Lane 2) 1X PBS (negative control) using ab175199 at a 1/10 dilution and HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.