All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilutionLane 1 : Caco 2 cell lysateLane 2 : K562 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of HCT-116 cells (paraformaldehyde-fixed, 4%) labeling TXNL1 with ab188328 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/10000 dilution + 293 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (ab188328) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : RAW 264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human thyroid papillary adenocarcinoma tissue labeling TXNL1 with ab188328 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Flow Cytometric analysis of 293 cells (paraformaldehyde-fixed, 2%) labeling TXNL1 with ab188328 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.