All lanes : Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilutionLane 1 : Mouse testis lysateLane 2 : Rat testis lysateLane 3 : Jurkat cell lysateLane 4 : HeLa cell lysateLane 5 : K562 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).