Anti-Ube2N / Ubc13 antibody
| Name | Anti-Ube2N / Ubc13 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab25885 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N |
| Description | Rabbit Polyclonal |
| Gene | UBE2N |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis in human small intestine cell lysates 15ug/lane )Lane 1 : 0.5 ug/ml of antibody.Lane 2 : 1 ug/ml of antibody.Lane 3 : 2 ug/ml of antibody.
ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC
ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.