Immunohistochemistical detection of Anti-UBE2V1 antibody (ab88679; 1/50 for 1h @ 21°C) on human kidney formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 0.25% Casein in PBS for 10 mins at 21°C. Secondary antibody HRP conjugated Dako REAL EnVision Goat anti Rabbit/Mouse (undiluted).See Abreview
Anti-UBE2V1 antibody (ab88679) at 1 µg/ml + Human pancreas tissue lysate at 50 µg
All lanes : Anti-UBE2V1 antibody (ab88679) at 1 µg/mlLane 1 : UBE2V1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.
ab88679 at 10µg/ml staining UBE2V1 in HeLa cells by Immunofluorescence.