Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5]

• Supplier: Abcam
• Catalog: ab110252
• Prices: $391.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 16D10AD9AH5
• Applications: FC ICC/IF ICC/IF WB
• Species Reactivities: Mouse, Rat, Bovine, Human
• Antigen: Bovine Ubiquinol-Cytochrome C Reductase Core Protein I protein
• Description: Mouse Monoclonal
• Gene: UQCRC1
• Conjugate: Unconjugated

Product images

ab110252 stained HCT116 cells. The cells were 100% methanol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150117 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

All lanes : Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (ab110252) at 0.5 µg/mlLane 1 : Human heart mitochondrial lysateLane 2 : Cow heart mitochondrial lysateLane 3 : Rat heart mitochondrial lysateLane 4 : Mouse heart mitochondrial lysateLane 5 : HepG2 mitochondrial lysate

Overlay histogram showing HepG2 cells stained with ab110252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110252, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Product References

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