All lanes : Anti-UBQLN2 antibody [6H9] (ab190283) at 1/1000 dilutionLane 1 : Primary mouse neuron and glia cell culturesLane 2 : Primary mouse neuron and glia cells transduced with Human ubiquilin 2 wild typeLane 3 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P506T mutantLane 4 : Primary mouse neuron and glia cells transduced with ubiquilin 2 P497S mutantLane 5 : Primary mouse neuron and glia cell cultures transduced with enhanced GFP controlLane 6 : HeLa cell lysateLane 7 : 3T3 cell lysate