All lanes : Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) dilution followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Anti-UCHL5IP antibody [EPR15341-56] (ab192616) at 1/1000 dilution + RAW 264.7 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab192616 at 1/150 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling UCHL5IP with ab192616 at 1/500 dilution (5μg/ml) followed by pre-diluted HRP Polymer for Rabbit/Mouse IgG secondary antibody and counter-stained with Hematoxylin. (inset: negative control).
Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling UCHL5IP with ab192616 at 1/480 dilution (red) or a rabbit IgG (negative) (black), unlabeled cells (blue) followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.