Anti-UCP2 antibody
| Name | Anti-UCP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab97931 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Mouse, Rat, Hamster, Cat, Dog, Pig, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human UCP2 |
| Description | Rabbit Polyclonal |
| Gene | UCP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-UCP2 antibody (ab97931) at 1 µg/mlLane 1 : Adipose (Human) Total Protein Lysate - adult normal tissue (ab28980)Lane 2 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
IHC image of ab97931 staining in human lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97931, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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Watanabe N, Inagawa K, Shibata M, Osakabe N. Lipids Health Dis. 2014 Apr 5;13:64.
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Nagy L, Docsa T, Szanto M, Brunyanszki A, Hegedus C, Marton J, Konya B, Virag L, Somsak L, Gergely P, Bai P. PLoS One. 2013 Jul 25;8(7):e69420.