All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/20000 dilutionLane 1 : Human fetal liver lysateLane 2 : A549 cell lysateLane 3 : U-87 MG cell lysateLane 4 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1000 µg
All lanes : Anti-UFC1 antibody [EPR15014] (ab189251) at 1/5000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : PC12 cell lysateLane 4 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling UFC1 with ab189251 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.