Anti-UFC1 antibody [EPR15014-102]

• Supplier: Abcam
• Catalog: ab189252
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15014-102
• Applications: IP ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Mouse, Rat, Human
• Antigen: Recombinant fragment within Human UFC1 aa 1-150
• Description: Rabbit Monoclonal
• Gene: UFC1
• Conjugate: Unconjugated

Product images

All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/20000 dilutionLane 1 : Human fetal liver lysateLane 2 : U87-MG cell lysateLane 3 : MCF7 cell lysateLane 4 : A549 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

All lanes : Anti-UFC1 antibody [EPR15014-102] (ab189252) at 1/5000 dilutionLane 1 : RAW 264.7 cell lysateLane 2 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling UFC1 with ab189252 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Immunofluorescent analysis of MCF7 cells(4% paraformaldehyde-fixed)  labeling UFC1 with ab189252 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot analysis of immunoprecipitation pellet from MCF7cell lysate immunoprecipitated using ab189252 at 1/50 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.