All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal heart lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilutiondeveloped using the ECL technique
All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/1000 dilutionLane 1 : Mouse spleen lysateLane 2 : rat C6 cell lysateLane 3 : mouse Raw 264.7 cell lysateLane 4 : mouse NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human thyroid carcinoma tissue labeling UFSP2 with ab192597 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.
Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling UFSP2 with ab192597 at a 1/100 dilution. A secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.