All lanes : Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilutionLane 1 : Human fetal liver lysateLane 2 : Human fetal kidney lysateLane 3 : Human testis lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilutiondeveloped using the ECL technique
Immunofluorescence analysis of, paraformaldehyde-fixed, HepG2 cells labeling UGT3A1 with ab181245 at a 1/500 dilution. As secondary antibody goat anti rabbit IgG (Alexa Fluor®555) was used at a 1/200. In blue DAPI staining.
Anti-UGT3A1 antibody [EPR13723] (ab181245) at 1/1000 dilution + HepG2 cell lysate at 10 µgSecondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling UGT3A1 with ab181245 at a 1/100 dilution. Counter stained with hematoxylin.
Western blot analysis on immunoprecipitation from 1) Human fetal liver lysate or 2) negative control, labeling UGT3A1 using ab181245 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG at a 1/1500 dilution.