
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab126478 1-4 µg/ml. Cells treated with PFA/Triton X-100.
![Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18186_ab126478-2-HPA044405.jpg)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

ab126478, at 1/50 dilution, staining UPF0532 in paraffin embedded Human kidney tissue by Immunohistochemistry.