All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/20000 dilutionLane 1 : HeLa cell lysateLane 2 : Human fetal heart lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-UQCRB antibody [EPR15591] (ab190360) at 1/5000 dilution + 293 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-UQCRB antibody [EPR15591] (ab190360) at 1/1000 dilutionLane 1 : Mouse heartLane 2 : Rat brainLane 3 : NIH-3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adenocarcinoma of the colon labeling UQCRB with ab190360 at 1/100 and HRP Polymer for Rabbit IgG. Counterstained with hematoxylin. Negative control also shown.
Immunocytochemical analysis of HeLa cells fixed in 4% paraformaldehyde labeling UQCRB with ab190360 at 1/250 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Counterstained with DAPI. Negative controls also shown.