ICC/IF image of ab103616 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103616 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Human colon tissue lysate at 50 µg
Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg
Anti-UQCRC2 antibody (ab103616) at 5 µg/ml + A431 cell lysate at 50 µg
All lanes : Anti-UQCRC2 antibody (ab103616) at 5 µg/mlLane 1 : UQCRC2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.