Anti-UQCRFS1 [EPR16289] antibody - C-terminal

• Supplier: Abcam
• Catalog: ab191079
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16289
• Applications: IHC-P ICC/IF ICC/IF IP FC WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UQCRFS1 aa 200 to the C-terminus
• Description: Rabbit Monoclonal
• Gene: UQCRFS1
• Conjugate: Unconjugated

Product images

Western blot analysis of immunoprecipitation pellet from Human fetal heart lysate immunoprecipitated using ab191079 at 1/90 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling UQCRFS1with ab191079 at 1/280 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunofluorescent analysis of HepG2 cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100 permeabilized) labeling UQCRFS1 with ab191079 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). Negative controls also shown.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1 with ab191079 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).

All lanes : Anti-UQCRFS1 [EPR16289] antibody - C-terminal (ab191079) at 1/1000 dilutionLane 1 : PC12 cell lysateLane 2 : Mouse brain lysateLane 3 : Mouse brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution

All lanes : Anti-UQCRFS1 [EPR16289] antibody - C-terminal (ab191079) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : Raji cell lysateLane 3 : 293 cell lysateLane 4 : Human fetal heart lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugate at 1/1000 dilution