Anti-UQCRFS1 antibody [5A5]
| Name | Anti-UQCRFS1 antibody [5A5] |
|---|---|
| Supplier | Abcam |
| Catalog | ab14746 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | 5A5 |
| Applications | WB ICC/IF FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Bovine, Human |
| Antigen | Bovine Complex III |
| Description | Mouse Monoclonal |
| Gene | UQCRFS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 0.5 µg/mlLane 1 : Hela Whole Cell Lysate - Hydroxyurea TreatedLane 2 : Hela Whole Cell Lysate - Bleomycin Treated Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated Lysates/proteins at 10 µg per lane.SecondaryMouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 25 kDa (why is the actual band size different from the predicted?)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18286_UQCRFS1-Primary-antibodies-ab14746-3.jpg)
All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 0.5 µg/mlLane 1 : Hela Whole Cell Lysate - Hydroxyurea TreatedLane 2 : Hela Whole Cell Lysate - Bleomycin Treated Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated Lysates/proteins at 10 µg per lane.SecondaryMouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionObserved band size : 25 kDa (why is the actual band size different from the predicted?)
Human normal kidney (cortex). Staining is observed intracellular. Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
![All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 1 µg/mlLane 1 : Isolated mitochondria from human heart at 5 µgLane 2 : Isolated mitochondria from bovine heart at 1 µgLane 3 : Isolated mitochondria from rat heart at 10 µgLane 4 : Isolated mitochondria from mouse heart at 10 µg](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18288_UQCRFS1-Primary-antibodies-ab14746-5.jpg)
All lanes : Anti-UQCRFS1 antibody [5A5] (ab14746) at 1 µg/mlLane 1 : Isolated mitochondria from human heart at 5 µgLane 2 : Isolated mitochondria from bovine heart at 1 µgLane 3 : Isolated mitochondria from rat heart at 10 µgLane 4 : Isolated mitochondria from mouse heart at 10 µg
ab24746 staining UQCRFS1 in murine cardiac myocytes by Immunocytochemistry/ Immunofluorescence.Cells were fixed in 4% paraformaldehyde and permeabilized using 0.1% Triton X100. Samples were blocked using 10% horse serum for 1 hour at room temperature then incubated with ab14746 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor488 conjugated goat anti-mouse IgG, used at a 1/500 dilution.See Abreview
![Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/18290_UQCRFS1-Primary-antibodies-ab14746-12.jpg)
Overlay histogram showing Raji cells stained with ab14746 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14746, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Complex I function and supercomplex formation are preserved in liver mitochondria - Complex I function and supercomplex formation are preserved in liver mitochondria
Davoudi M, Kotarsky H, Hansson E, Fellman V. PLoS One. 2014 Jan 22;9(1):e86767.
A stabilizing factor for mitochondrial respiratory supercomplex assembly - A stabilizing factor for mitochondrial respiratory supercomplex assembly
Ikeda K, Shiba S, Horie-Inoue K, Shimokata K, Inoue S. Nat Commun. 2013;4:2147.
Mutations in LYRM4, encoding iron-sulfur cluster biogenesis factor ISD11, cause - Mutations in LYRM4, encoding iron-sulfur cluster biogenesis factor ISD11, cause
Lim SC, Friemel M, Marum JE, Tucker EJ, Bruno DL, Riley LG, Christodoulou J, Kirk EP, Boneh A, DeGennaro CM, Springer M, Mootha VK, Rouault TA, Leimkuhler S, Thorburn DR, Compton AG. Hum Mol Genet. 2013 Nov 15;22(22):4460-73.
Widespread and enzyme-independent Nepsilon-acetylation and Nepsilon-succinylation - Widespread and enzyme-independent Nepsilon-acetylation and Nepsilon-succinylation
Wagner GR, Payne RM. J Biol Chem. 2013 Oct 4;288(40):29036-45.
The pyrimidine nucleotide carrier PNC1 and mitochondrial trafficking of thymidine - The pyrimidine nucleotide carrier PNC1 and mitochondrial trafficking of thymidine
Franzolin E, Miazzi C, Frangini M, Palumbo E, Rampazzo C, Bianchi V. Exp Cell Res. 2012 Oct 15;318(17):2226-36.
A mild impairment of mitochondrial electron transport has sex-specific effects on - A mild impairment of mitochondrial electron transport has sex-specific effects on
Hughes BG, Hekimi S. PLoS One. 2011;6(10):e26116.
Mutations in iron-sulfur cluster scaffold genes NFU1 and BOLA3 cause a fatal - Mutations in iron-sulfur cluster scaffold genes NFU1 and BOLA3 cause a fatal
Cameron JM, Janer A, Levandovskiy V, Mackay N, Rouault TA, Tong WH, Ogilvie I, Shoubridge EA, Robinson BH. Am J Hum Genet. 2011 Oct 7;89(4):486-95.
Characterization of complex III deficiency and liver dysfunction in GRACILE - Characterization of complex III deficiency and liver dysfunction in GRACILE
Kotarsky H, Karikoski R, Morgelin M, Marjavaara S, Bergman P, Zhang DL, Smet J, van Coster R, Fellman V. Mitochondrion. 2010 Aug;10(5):497-509.
Long-term survival of neonatal mitochondrial complex III deficiency associated - Long-term survival of neonatal mitochondrial complex III deficiency associated
Tuppen HA, Fehmi J, Czermin B, Goffrini P, Meloni F, Ferrero I, He L, Blakely EL, McFarland R, Horvath R, Turnbull DM, Taylor RW. Mol Genet Metab. 2010 Aug;100(4):345-8.
Mutations in mitochondrial complex III uniquely affect complex I in - Mutations in mitochondrial complex III uniquely affect complex I in
Suthammarak W, Morgan PG, Sedensky MM. J Biol Chem. 2010 Dec 24;285(52):40724-31.