All lanes : Anti-UQCRFS1 antibody [EPR16288] (ab191078) at 1/10000 dilutionLane 1 : HeLa lysateLane 2 : Raji lysatesLane 3 : 293 lysatesLane 4 : Human fetal heart lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-UQCRFS1 antibody [EPR16288] (ab191078) at 1/1000 dilutionLane 1 : mouse brain lysateLane 2 : mouse heart lysateLane 3 : mouse kidney lysateLane 4 : rat brain lysateLane 5 : rat heart lysateLane 6 : rat kidney lysateLane 7 : RAW264.7 lysateLane 8 : NIH3T3 lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of UQCRFS1 in immunoprecipitation pellets from Human fetal lysate. ab191078 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1500 dilution.
Immunofluorescent analysis of methanol-fixed HeLa cells labeling UQCRFS1 with ab191078 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 and DAPI staining (blue). Negative controls also shown.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling UQCRFS1 with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue labeling UQCRFS1 with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling UQCRFS1 with ab191078 at 1/500. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.