ab121292 at 1/75 dilution staining URB2 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
ab121292 at 4 µg/ml staining URB2 in PFA/Triton X-100 fixed/permeabilized U 2 OS cells by Immunofluorescence (green).
All lanes : Anti-URB2 antibody (ab121292) at 1/1000 dilutionLane 1 : Hela extracts 25ugLane 2 : RPE-1 extracts 25ugLane 3 : Hela extracts 50ugLane 4 : RPE-1 extracts 50ugSecondaryDonkey LICOR anti-rabbit IRDye680 at 1/20000 dilutionPerformed under reducing conditions.Observed band size : 130 kDa (why is the actual band size different from the predicted?)Additional bands at : 39 kDa (possible cross reactivity).Exposure time : 3 minutesSee Abreview