All lanes : Anti-URB2 antibody [EPR13404-35] (ab181177) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : Raji cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling URB2 with ab181177 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).