Western blot anlaysis of extracts from NCI-H295R cells, MLTC-1 cells, and various tissues using CYP11A1 (D8F4F) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CYP11A1 protein (hCYP11A1-Myc/DDK; +), using CYP11A1 (D8F4F) Rabbit mAb.
Western blot analysis of extracts from NCI-H295R (positive) and SW-13 (negative) cells using CYP11A1 (D8F4F) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Immunoprecipitation of CYP11A1 from NCI-H295R cell extracts, using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or CYP11A1 (D8F4F) Rabbit mAb. Lane 1 is 10% input. Western blot analysis was performed using CYP11A1 (D8F4F) Rabbit mAb.
Confocal immunofluorescent analysis of NCI-H295R (left, positive) and SW-13 (right, negative) cells, using CYP11A1 (D8F4F) Rabbit mAb (green). Actin filaments were labeled with DyLight ® 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).