All lanes : Anti-USH1C antibody [EPR8131] (ab133763) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human colon lysateLane 3 : Caco 2 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP conjugated antibody at 1/2000 dilution
All lanes : Anti-USH1C antibody [EPR8131] (ab133763) at 1/2500 dilutionLane 1 : Squirrel cochleaLane 2 : Mouse cochleaLane 3 : Squirrel colonLane 4 : Mouse colonLane 5 : Caco2 cellsSecondaryHRP linked Goat anti-rabbit IgG polyclonal at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD