Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling USP36 with ab72243 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-USP36 antibody (ab72243) at 0.4 µg/mlLane 1 : Whole cell lysate from Hela cells at 50 µgLane 2 : Whole cell lysate from Hela cells at 15 µgLane 3 : Whole cell lysate from Hela cells at 5 µgLane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation/ Western Blot of USP36. Lane 1: ab72243 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72243 at 1µg/ml for subsequent WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Detection:Chemiluminescence with an exposure time of 3 minutes.