All lanes : Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal (ab181175) at 1/5000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Ramos cell lysateLane 4 : K562 cell lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling valyl tRNA synthetase with ab181175 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling valyl tRNA synthetase with ab181175 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).