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All lanes : Anti-VASH1 antibody (ab67423) at 1/500 dilutionLane 1 : VASH1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg/ml per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution