Anti-VASH1 antibody [EPR17420]

• Supplier: Abcam
• Catalog: ab199732
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17420
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VASH1 aa 250-350
• Description: Rabbit Monoclonal
• Gene: VASH1
• Conjugate: Unconjugated

Product images

All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/5000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysateLane 3 : A431 (Human epidermoid carcinoma) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/1000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Mouse kidney lysateLane 4 : Rat brain lysateLane 5 : Rat heart lysateLane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysateLane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/1000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal spleen lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

VASH1 was immunoprecipitated from 1mg of A431 (Human epidermoid carcinoma) whole cell lysate with ab199732 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199732 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: A431 whole cell lysate 10 µg (Input). Lane 2: ab199732 IP in A431 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199732 in A431 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.