Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control
| Name | Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control |
|---|---|
| Supplier | Abcam |
| Catalog | ab15895 |
| Prices | $401.00, $803.00 |
| Sizes | 100 µg, 250 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Chicken, Dog, Human, Zebrafish, Hamster, Rabbit, Bovine, Pig |
| Antigen | Synthetic peptide derived from residues 150 - 250 of Human VDAC1/ Porin |
| Blocking Peptide | Human VDAC1 / Porin peptide |
| Description | Rabbit Polyclonal |
| Gene | VDAC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/mlLane 1 : Hela Nuclear lysateLane 2 : Hela cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysateLane 5 : HEK293 cell lysateLane 6 : Hela Nuclear lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/mlLane 7 : Hela cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/mlLane 8 : A431 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/mlLane 9 : Jurkat cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/mlLane 10 : HEK293 cell lysate with Human VDAC1 / Porin peptide (ab16131) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab15895 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15895, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
Image courtesy of Human Protein AtlasParaffin embedded sections of human kidney were incubated with ab15895 (1/600 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab15895 was also tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/mlLane 1 : Heart (Mouse) Tissue LysateLane 2 : Kidney (Mouse) Tissue LysateLane 3 : Skeletal Muscle (Mouse) Tissue Lysate (ab29711)Lane 4 : Spinal Cord (Mouse) Tissue LysateLane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate Lane 6 : Brain (Rat) Tissue Lysate - normal tissueLane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)Lysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.
ab15895 staining mouse heart tissue section by IHC-P. Sections were PFA fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in 10% serum for 10 minutes at 25°C. The primary antibody was diluted 1/600 and incubated with the sample for 1 hour at 25°C. A biotinylated goat anti-rabbit antibody diluted 1/400 was used as the secondary.See Abreview
All lanes : Anti-VDAC1 / Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/mlLane 1 : Adult wild type zebrafish brain mitochondrial lysate at 20 µgLane 2 : Adult wild type zebrafish brain cytosolic lysate at 30 µgSecondaryHRP-conjugated goat F(ab’)2 Fragment at 1/10000 dilutiondeveloped using the ECL technique
ab15895 staining VDAC1 / Porin in human cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then incubated with ab15895 at a 1/1000 dilution for 12 hours at 4°C. The secondary used was an undiluted HRP conjugated anti-rabbit polyclonal. Note: The big cells are purkinje cells of the cerebellum, (no nuclear staining).See Abreview
developed using the ECL techniquePerformed under reducing conditions.
developed using the ECL techniquePerformed under reducing conditions.
Product References
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