Anti-VDAC2 antibody
| Name | Anti-VDAC2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77160 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB ELISA FC |
| Species Reactivities | Human |
| Antigen | GST-tagged recombinant full length Human protein (AAH00165) |
| Description | Mouse Monoclonal |
| Gene | VDAC2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing HepG2 cells stained with ab77160 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77160, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/19389_ab77160-3-ab77160FC.jpg)
Overlay histogram showing HepG2 cells stained with ab77160 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77160, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Anti-VDAC2 antibody (ab77160) at 1 µg/ml + GST-tagged recombinant full length Human VDAC2 at 0.1 µgSecondaryGoat Anti-Mouse IgG (H&L) HRP.conjugated at 1/5000 dilution
All lanes : Anti-VDAC2 antibody (ab77160) at 5 µg/mlLane 1 : VDAC2-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution
Product References
ER stress activates the NLRP3 inflammasome via an UPR-independent pathway. - ER stress activates the NLRP3 inflammasome via an UPR-independent pathway.
Menu P, Mayor A, Zhou R, Tardivel A, Ichijo H, Mori K, Tschopp J. Cell Death Dis. 2012 Jan 26;3:e261.
A role for mitochondria in NLRP3 inflammasome activation. - A role for mitochondria in NLRP3 inflammasome activation.
Zhou R, Yazdi AS, Menu P, Tschopp J. Nature. 2011 Jan 13;469(7329):221-5.