Anti-Versican antibody [EPR12277]

• Supplier: Abcam
• Catalog: ab177480
• Prices: $378.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12277
• Applications: IHC-P WB
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Versican aa 3200-3300 (Cysteine residue)
• Description: Rabbit Monoclonal
• Gene: VCAN
• Conjugate: Unconjugated

Product images

All lanes : Anti-Versican antibody [EPR12277] (ab177480) at 1/1000 dilutionLane 1 : Fetal brain lysateLane 2 : Fetal heart lysateLysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Versican with ab177480 at 1/50 dilution.

ab177480 staining Versican in mouse cortex and hippocampus tissue section by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X and blocked with 3% BSA for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in PBS + 2% BSA + Triton X) for 18 hours at 4°C. A HRP-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.See Abreview