All lanes : Anti-VPS11 antibody [EPR10344(2)] (ab189920) at 1/10000 dilutionLane 1 : Human fetal brain lysateLane 2 : K562 cell lysateLane 3 : 293 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-VPS11 antibody [EPR10344(2)] (ab189920) at 1/1000 dilution + Raw264.7 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling VPS11 with ab189920 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling VPS11 with ab189920 at 1/90 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.