Anti-VR1 antibody

• Supplier: Abcam
• Catalog: ab10295
• Prices: $378.00
• Sizes: 50 µl
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F ICC/IF ICC/IF
• Species Reactivities: Rat, Guinea Pig
• Antigen: Synthetic peptide: YTGSLKPEDAEVFKDSMVPGEK , corresponding to C terminal amino acids 817-838 of Rat Vanilloid Receptor 1
• Blocking Peptide: VR1 peptide
• Description: Polyclonal
• Gene: Trpv1
• Conjugate: Unconjugated

Product images

ab10295 stained PC12 cells. The cells were 100% Methanol fixed for 5 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10295 at 1in100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab96959 Dylight 488 goat anti Guinea pig used at a 1/250 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

ab10295, at a dilution of 1/1000, staining VR1 in rat dorsal root ganglion by Immunohistochemistry (PFA perfusion fixed frozen sections).

Product References

Functional interaction between TRPV1 and mu-opioid receptors in the descending - Functional interaction between TRPV1 and mu-opioid receptors in the descending

Maione S, Starowicz K, Cristino L, Guida F, Palazzo E, Luongo L, Rossi F, Marabese I, de Novellis V, Di Marzo V. J Neurophysiol. 2009 May;101(5):2411-22.

An N-terminal variant of Trpv1 channel is required for osmosensory transduction. - An N-terminal variant of Trpv1 channel is required for osmosensory transduction.

Sharif Naeini R, Witty MF, Seguela P, Bourque CW. Nat Neurosci. 2006 Jan;9(1):93-8. Epub 2005 Dec 4.