All lanes : Anti-WAPL antibody [EPR3407(2)] (ab109537) at 1/50000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
ab109537 at 1/100 dilution staining WAPL in HeLa cells by Immunofluorescence.
All lanes : Anti-WAPL antibody [EPR3407(2)] (ab109537) at 1/10000 dilutionLane 1 : Human U2OS cells treated with siRNA control.Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL. Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL.Lysates/proteins at 90 µg per lane.Secondary Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.