Anti-WDR5 antibody
| Name | Anti-WDR5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22512 |
| Prices | $392.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Zebrafish, Chicken, Bovine, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human WDR5 |
| Blocking Peptide | Human WDR5 peptide |
| Description | Rabbit Polyclonal |
| Gene | WDR5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-WDR5 antibody (ab22512) at 1 µg/ml (blocked with 5% Milk)Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell LysateLane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab22512 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab22512 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-WDR5 antibody (ab22512) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Biochemical reconstitution and phylogenetic comparison of human SET1 family core - Biochemical reconstitution and phylogenetic comparison of human SET1 family core
Shinsky SA, Monteith KE, Viggiano S, Cosgrove MS. J Biol Chem. 2015 Mar 6;290(10):6361-75.
Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and - Mixed lineage leukemia 5 (MLL5) protein regulates cell cycle progression and
Zhou P, Wang Z, Yuan X, Zhou C, Liu L, Wan X, Zhang F, Ding X, Wang C, Xiong S, Wang Z, Yuan J, Li Q, Zhang Y. J Biol Chem. 2013 Jun 14;288(24):17532-43.
Gene dysregulation by histone variant H2A.Z in bladder cancer. - Gene dysregulation by histone variant H2A.Z in bladder cancer.
Kim K, Punj V, Choi J, Heo K, Kim JM, Laird PW, An W. Epigenetics Chromatin. 2013 Oct 16;6(1):34.
Rescue of the genetically engineered Cul4b mutant mouse as a potential model for - Rescue of the genetically engineered Cul4b mutant mouse as a potential model for
Chen CY, Tsai MS, Lin CY, Yu IS, Chen YT, Lin SR, Juan LW, Chen YT, Hsu HM, Lee LJ, Lin SW. Hum Mol Genet. 2012 Oct 1;21(19):4270-85.
The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and - The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and
Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey TN, Meyer B, Varagnolo L, Muller AM, Karas M, Dingermann T, Marschalek R. Leukemia. 2011 Jan;25(1):135-44.
Role of ZBP-89 in human globin gene regulation and erythroid differentiation. - Role of ZBP-89 in human globin gene regulation and erythroid differentiation.
Woo AJ, Kim J, Xu J, Huang H, Cantor AB. Blood. 2011 Sep 29;118(13):3684-93.
X-linked mental retardation gene CUL4B targets ubiquitylation of H3K4 - X-linked mental retardation gene CUL4B targets ubiquitylation of H3K4
Nakagawa T, Xiong Y. Mol Cell. 2011 Aug 5;43(3):381-91.
Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA - Roles for common MLL/COMPASS subunits and the 19S proteasome in regulating CIITA
Koues OI, Mehta NT, Truax AD, Dudley RK, Brooks JK, Greer SF. Epigenetics Chromatin. 2010 Feb 4;3(1):5.
Phosphorylation of MLL by ATR is required for execution of mammalian S-phase - Phosphorylation of MLL by ATR is required for execution of mammalian S-phase
Liu H, Takeda S, Kumar R, Westergard TD, Brown EJ, Pandita TK, Cheng EH, Hsieh JJ. Nature. 2010 Sep 16;467(7313):343-6.
DDB1-CUL4 and MLL1 mediate oncogene-induced p16INK4a activation. - DDB1-CUL4 and MLL1 mediate oncogene-induced p16INK4a activation.
Kotake Y, Zeng Y, Xiong Y. Cancer Res. 2009 Mar 1;69(5):1809-14.