Anti-WISP2 antibody
| Name | Anti-WISP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38317 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB ELISA |
| Species Reactivities | Human |
| Antigen | A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 127~141 of human WISP2 |
| Description | Rabbit Polyclonal |
| Gene | WISP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-WISP2 antibody (ab38317) at 1/100 dilution + A549 cell lysate
ICC/IF image of ab38317 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38317, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-WISP2 antibody (ab38317) at 1/100 dilution + Active human WISP2 full length protein (ab50040) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds
Product References
CCN5, a novel transcriptional repressor of the transforming growth factor beta - CCN5, a novel transcriptional repressor of the transforming growth factor beta
Sabbah M, Prunier C, Ferrand N, Megalophonos V, Lambein K, De Wever O, Nazaret N, Lachuer J, Dumont S, Redeuilh G. Mol Cell Biol. 2011 Apr;31(7):1459-69.
Role of WISP-2/CCN5 in the maintenance of a differentiated and noninvasive - Role of WISP-2/CCN5 in the maintenance of a differentiated and noninvasive
Fritah A, Saucier C, De Wever O, Bracke M, Bieche I, Lidereau R, Gespach C, Drouot S, Redeuilh G, Sabbah M. Mol Cell Biol. 2008 Feb;28(3):1114-23. Epub 2007 Dec 10.