Anti-Wnt1 antibody

• Supplier: Abcam
• Catalog: ab15251
• Prices: $401.00
• Sizes: 500 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ELISA IHC-P IHC-F WB
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (N terminal)
• Description: Rabbit Polyclonal
• Gene: WNT1
• Conjugate: Unconjugated

Product images

ab15251 staining Wnt1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

ICC/IF image of ab15251 stained mouse 3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15251, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

Breast carcinoma cells stained with ab15251 (Immunohistochemistry, paraffin sections);

Anti-Wnt1 antibody (ab15251) at 1/25 dilution + NIH3T3 cell lysate

Product References

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