Anti-Wnt1 antibody [10C8]

• Supplier: Abcam
• Catalog: ab91191
• Prices: $384.00
• Sizes: 100 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 10C8
• Applications: WB ELISA ICC/IF ICC/IF IHC-P
• Species Reactivities: Mouse, Human
• Antigen: Purified recombinant fragment of Wnt1 expressed in E
• Description: Mouse Monoclonal
• Gene: WNT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Wnt1 antibody [10C8] (ab91191) at 1/500 dilutionLane 1 : NIH3T3 cell lysateLane 2 : 3T3L1 cell lysateLane 3 : HeLa cell lysate

ab91191, staining Wnt1 in Hela (left) and 3T3L1 (right) cells by Confocal Immunofluorescence (green). Red: Actin filaments that have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

ab91191 staining Wnt1 in HepG2 cells by Confocal Immunofluorescence (green). Red: Actin filaments that have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

IHC image of Wnt1 staining in Human normal skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91191, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.